Lane 1 : Jurkat (Human) Whole Cell LysateLane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
SecondaryAll lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDaObserved band size: 120 kDaAdditional bands at: 20 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab24170 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.